Vol 3-2 Commentary

Curcumin, an Inhibitor of PAK1, Potential Treatment for COVID-19

Mohadeseh Nemati1, Elmira Roshani Asl1, Fahima Danesh Pouya1, Yousef Rasmi1,2*

1Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran

DOI: 10.29245/2689-9981/2020/2.1160 View / Download Pdf
Vol 3-2 Research Article

Retrospective Study of Tegumentary Leishmaniasis in Burkina Faso, from 2012 to 2016

Patindoilba Marcel Sawadogo1,2*, Kiswendsida Thierry Guiguemdé2,4, Adama Zida1,2, Nina korsaga/Somé2,3, Ibrahim Sangaré5,6, Sanata Bamba5,6, Salimata Kiemdé1, Rasmata Ouédraogo/Traoré2,4

1Parasitology-Mycology Service, Yalgado Ouédraogo University Hospital Center, Ouaga, Burkina Faso

2Training and Research Unit in Health Sciences, Ouaga University 1 Professor Joseph Ki-Zerbo (UO1 / PrZKZ), Ouagadougou, Burkina Faso

3Dermatology-Venereology service, Yalgado Ouédraogo University Hospital Center, Ouaga, Burkina Faso

4Parasitology-Mycology Service, Charles de Gaulle University Hospital Center, Ouagadougou, Burkina Faso

5Institute of Health Sciences, Nazi Boni University, Bobo Dioulasso, Burkina Faso

6Parasitology-Mycology Service, Souro Sanou University Hospital Center, Bobo Dioulasso, Burkina Faso

Background: In Burkina Faso, the first cases of tegumentary leismaniasis were reported in 1960. But it was not until the 1990s that the disease was really known by the Burkinabè with the epidemic that Ouagadougou experienced at that time. Since 2000, the disease has become neglected. However, the frequency of cases diagnosed in hospitals suggests that tegumentary leismaniasis is still endemic in Ouagadougou. Hence the present study whose purpose and assess the current state of the disease in the city of Ouagadougou from 2012 to 2016.

Methods: We conducted a descriptive study on retrospective data collected from January 2012 to December 2016 in different hospitals in the city of Ouagadougou. Data were collected from clinical and laboratory registers.

Results: a total of 96 parasitologically-confirmed cases were identified. Clinical forms were mentioned in 43 patients. Localized cutaneous leishmaniasis was the most common form 25/43 (58.14%), followed by mucocutaneous leishmaniasis 8/43 (18.60%), diffuse cutaneous leishmaniasis 7/43 (16.28%) and finally pseudo-leprosy Leishmaniasis 3/43 (6.98%). The Leishmaniasis / HIV association was found in 15 patients/96 (15.63%). The parasitological examination in search of amastigote forms had a positivity rate of 92/209 (44.02%). Meglumine antimoniate (Glucantime®), the WHO reference drug, was the most prescribed 35/42 (83.33%).

Conclusion: Tegumentary Leishmaniasis still exists in Ouagadougou. Thus, precautions must be taken to avoid an upsurge of cutaneous Leishmaniasis in Ouagadougou.

DOI: 10.29245/2689-9981/2020/2.1159 View / Download Pdf
Vol 3-2 Research Article

Investigation on Influenza A and B Virus from People Entering China via the Tianjin Port in 2019

Biyu1†, Pang Zheng2†, Shao Lijun1, Tang Yunfeng1, Niu Guoyu1*

1School of Public Health, WeiFang Medical University, China

2Tianjin International Joint Academy of Biomedicine, Tianjin, China

Objective: To investigate the frequency of influenza A and influenza B infections in people entering China at Tianjin port during 2019, so as to provide the basis for preventing the spread and control of influenza A and influenza B virus in China, and preliminarily assess the risk of entry passengers.

Methods: The throat swabs of entry personnel at Tianjin port in 2019 were collected, and influenza A and influenza B virus in the samples were detected by quantitative RT-PCR. According to the collected passenger information, combined with the experimental results, classification was carried out, and the related factors influencing the positive of influenza A and influenza B virus were analyzed.

Results: A total of 1605 throat swabs were collected. The results of quantitative RT-PCR showed that there were 40 (2.5%) cases of influenza A virus positive. No significant differences were found in gender, age and country distribution. However, in terms of entry time, the positive rate of influenza A virus was the highest in the first quarter compared with other quarters, and the difference was statistically significant. At the same time, there were 13 (0.8%) cases of influenza B virus positive in this survey. No significant differences were found in gender, country distribution and entry time, but in terms of the age of entry personnel, the positive detection rate of children and young people with influenza B infection was higher, and the difference between different age groups was statistically significant.

Conclusions: In 2019, Chinese accounted for the majority of the entry personnel at Tianjin port. The detection rates of influenza A and influenza B virus were low, while the total number of foreign immigrants was small, and the detection rate was high. The positive rate of influenza A and influenza B among different genders was similar. The positive detection rate of influenza A virus in the first quarter and influenza B virus in adolescents were relatively high. These two groups of people posed a threat to public health safety in China. Therefore, Therefore, we should focus on prevention and monitoring of key population at ports.

View / Download Pdf
Vol 3-2 Original Research Article

COVID-19 Disease as an Acute Angiotensin 1-7 Deficiency: A Preliminary Phase 2 Study with Angiotensin 1-7 in Association with Melatonin and Cannabidiol in Symptomatic COVID19 -Infected Subjects

Paolo Lissoni1*, Franco Rovelli1, Alejandra Monzon1, Giusy Messina1, Enrica Porta1, Giorgio Porro1, Sonia Pensato1, Elio Martin1, Andrea Sassola1, Alberto Caddeo1, Carla Galli1, Nicoletta Merli1, Agnese Valentini1, Giuseppe Di Fede1

1Institute of Biological Medicine, Milan, Italy

COVID-19 disease is characterized by severe and acute immune alterations, consisting of an abnormal secretion of inflammatory cytokines, mainly IL-17, IL-6 and TNF-alpha, in association with decline in lymphocyte and increase in monocyte counts. ACE2 is the key for COVID19 entry into the cells. However, the link of viral spike protein to ACE2 receptor on cell surface would also block the ACE2 enzymatic activity itself, with a consequent diminished production of angiotensin 1-7 (Ang 1-7), which is provided by fundamental anti-inflammatory and anti-coagulant properties. Then, the severe and acute Ang 1-7 deficiency would allow an exaggerated cytokine-induced inflammatory response, endothelial damage, leak capillary syndrome and acute respiratory distress syndrome (ARDS). Moreover, because of the documented connections occurring among ACE2, cannabinoid system and melatonin (MLT) secretion from the pineal gland, the block of ACE2 activity would also allow a concomitant deficiency of pineal-cannabinoid system axis, which plays a fundamental anti-inflammatory role by inhibiting IL-17 secretion, one of the main cytokine involved in COVID19 infection. Therefore, COVID19-induced exaggerated inflammatory response could be controlled at least in part by correcting Ang 1-7, MLT and cannabinoid deficiency through their exogenous administration. On these bases, a study was planned in 30 COVID19-infected patients with initial or important symptomatology, 16 of whom orally treated by MLT (20 mg/day in the evening) plus cannabidiol (CBD) (10 mg x 2/day) only, while the other 14 patients received also Ang 1-7 (0.5 mg 2/day orally). The results were compared to those observed in a control group of 30 COVID-19 infected patient, who received the only supportive therapy. No hospitalisation for initial respiratory failure was required in the group of patients treated by the neuroimmune regimen. In addition, most patients referred a rapid disappearance of fever and myalgia, as well as a relief of asthenia, particularly in those concomitantly treated with Ang 1-7. On the contrary, 5/30 (17%) control patients required hospitalisation. The difference was statistically significant (0/30 vs 5/30, P< 0.05). This preliminary study would suggest that a neuroimmune approach consisting of MLT and CBD in association with Ang 1-7 is an effective and non-toxic regimen in the therapy of COVID19-related symptoms, which could also control the clinical evolution of disease and reduce the need of hospitalisation.

DOI: 10.29245/2689-9981/2018/2.1162 View / Download Pdf
Vol 4-1 Research Article

A Cross Sectional Study Comparing Blood Parameters for Short Term Adverse Outcome in Syncope Patients

Farizan Tajudin, Nik Azlan Nik Muhamad*

Emergency Department, Medical Centre, National University of Malaysia, Kuala Lumpur, Wilayah Persekutuan, Malaysia

Introduction: Mortality from syncope may not be apparent on initial evaluation in emergency department (ED). No reliable tools or clinical decision rule was established risk stratify mortality or morbidity from syncope. This study relates blood parameters with short term serious outcome of syncope. Methods: This was a single centre case control study of adults presenting to emergency department with syncope (n = 98), over 3 months’ duration. Patients particulars, contact profile and blood investigation results were recorded. Patients were called up to ascertain development of serious outcome of syncope within 7 days. Blood parameters such as hemoglobin, hematocrit, serum urea, serum sodium and Troponin I were compared between serious outcome and non-serious outcome group. Result: There was significant difference (p < 0.05) in hemoglobin, hematocrit, serum urea and Troponin I level between the 2 groups of syncope patients. Lower hemoglobin (11.5 ±3.4 g/dL vs. 13.0 ±2.1 g/dL) and lower hematocrit level (34.5 ±9.1 % vs. 38.8 ±5.8 %) were associated with development of short-term serious outcome after syncope. Higher serum urea (9.3 ±8.0 mmol/L vs. 4.3 ±1.2 mmol/L) and abnormally elevated Troponin I (x2 = 15.77; p < .001) were associated with development of short-term serious outcome after syncope. Conclusion: The parameters studied can be a baseline guide for disposition of syncope patient in ED. Further interventional prospective studies are required.

View / Download Pdf